simultaneous determination of ibuprofen and caffeine in urine samples by combining mcr-als and excitation-emission data

second order advantage of excitation-emission fluorescence matrix was applied for the simultaneous determination of ibuprofen and caffeine. the proposed method is based on the measurement of the native fluorescence and recording emission spectra of ibuprofen and caffeine in different excitation wavelengths. the mixture of these compounds was resolved by multivariate curve resolution coupled with alternative least squares (mcr-als) on constructed matrix. the eem spectra were recorded at excitation wavelengths from 250-275 nm; the emission wavelengths ranged from 275-400 nm. for each particular quantitative determination, an augmented matrix was defined. the resolution of each augmented-data matrix gave an estimation of the excitation and emission spectra of the species included in the model. ibuprofen and caffeine were determined in concentration range from 0.10-8.00 and 0.50-15.00 mg ml-1, respectively. the minimal sample pretreatment and relatively low running cost, make this method a good alternative to existing methods for determination of the analytes in urine samples.